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Autolytic activity and molecular characteristics of Staphylococcus haemolyticus strains with induced vancomycin resistance.
- Source :
-
Journal of medical microbiology [J Med Microbiol] 2012 Oct; Vol. 61 (Pt 10), pp. 1428-1434. Date of Electronic Publication: 2012 Jul 19. - Publication Year :
- 2012
-
Abstract
- The aim of this study was to investigate the molecular characteristics of induced vancomycin resistance in Staphylococcus haemolyticus. Autolytic properties and phenotypic characteristics of passage-selected vancomycin-resistant S. haemolyticus strains were examined. In addition, expression of autolysis-related genes (atl, lrgAB, sarA and lytS) was investigated using the RNase protection assay (RPA). The RPA results indicated that only the expression of the atl gene was significantly upregulated (2.5- to 6-fold increase) in vancomycin-intermediate and vancomycin-resistant strains. The vancomycin-resistant strains exhibited lower expression of murein hydrolase proteins and reduced autolytic activity compared with the parent strain. In addition, a reduced growth rate, cell wall thickening and higher survival rate in the presence of lysostaphin were observed in vancomycin-intermediate and vancomycin-resistant induced strains compared with the parent strain. In conclusion, altered autolytic properties, in particular upregulation of the atl gene, may contribute to vancomycin resistance in S. haemolyticus.
- Subjects :
- Autolysis
Bacterial Proteins genetics
Bacterial Proteins metabolism
Bacteriolysis genetics
Detergents pharmacology
Humans
Microscopy, Electron, Transmission
Mutation
Octoxynol pharmacology
Republic of Korea epidemiology
Reverse Transcriptase Polymerase Chain Reaction
Staphylococcal Infections epidemiology
Staphylococcal Infections microbiology
Staphylococcus haemolyticus genetics
Staphylococcus haemolyticus ultrastructure
Vancomycin Resistance genetics
Anti-Bacterial Agents pharmacology
Bacteriolysis physiology
Staphylococcus haemolyticus drug effects
Vancomycin pharmacology
Vancomycin Resistance physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1473-5644
- Volume :
- 61
- Issue :
- Pt 10
- Database :
- MEDLINE
- Journal :
- Journal of medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 22820692
- Full Text :
- https://doi.org/10.1099/jmm.0.041046-0