High expression of human monocyte iNOS mRNA induced by Paracoccidioides brasiliensis is not associated with increase in NO production.

Authors :: Bordon-Graciani AP
Dias-Melicio LA
Acorci-Valério MJ
Araujo JP Jr
Soares AM
Source :: Microbes and infection [Microbes Infect] 2012 Oct; Vol. 14 (12), pp. 1049-53. Date of Electronic Publication: 2012 Jul 20.
Publication Year :: 2012
Abstract: In this study we investigated the role of nitric oxide (NO) in monocyte fungicidal activity against Paracoccidioides brasiliensis. We found that cells primed with IFN-γ, TNF-α or GM-CSF and challenged with a high-(Pb18) or low-virulence (Pb265) strain of the fungus increase their fungicidal activity. Expression of iNOS mRNA was increased after priming cells with each cytokine, and tended to be inhibited by Pb18. Despite up-regulation of iNOS mRNA expression by Pb265, an equivalent increase in NO production was not detected, as metabolite levels were similar in all cultures. The results indicated that high expression of human monocyte iNOS mRNA induced by P. brasiliensis is not correlated with NO concentrations produced.<br /> (Copyright © 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)

Subjects :: Adult
Cells, Cultured
Gene Expression
Humans
Leukocytes, Mononuclear immunology
Leukocytes, Mononuclear microbiology
Microbial Viability
Middle Aged
Nitric Oxide Synthase Type II genetics
Young Adult
Host-Pathogen Interactions
Monocytes immunology
Monocytes microbiology
Nitric Oxide metabolism
Nitric Oxide Synthase Type II biosynthesis
Paracoccidioides immunology
RNA, Messenger biosynthesis

Full Text Access

Tools