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[Basophil CD63 expression in the blood of the anaphylactic shock rat].
- Source :
-
Fa yi xue za zhi [Fa Yi Xue Za Zhi] 2012 Jun; Vol. 28 (3), pp. 172-4, 178. - Publication Year :
- 2012
-
Abstract
- Objective: To explore the value of flow cytometry in anaphylactic shock diagnosis by CD63 expression being detected using flow cytometry to conform the activation of basophils.<br />Methods: Sixteen rats were randomly divided into two groups: control group and anaphylactic shock group. The model of anaphylactic shock rat with ovalbumin injection was established. CD63, CD45 and CD203c antibody combination, flow cytometry was employed to detected blood basophil CD63 expression. Immunofluorescence method was employed to observe the CD63 immunofluorescence staining in the rat lung tissue.<br />Results: (1) Pure basophils were obtained by CD45 and CD203c gating. (2) The percentages of basophils CD63 were (17.34 +/- 2.04)% and (1.52 +/- 0.35)% in the experimental and control group, respectively. The differences between two groups were statistically significant (P < 0.01). (3) Compared with the control group, the expression of CD63 in basophils increased in anaphylactic shock lung tissue.<br />Conclusion: The detection of CD63 by flow cytometry could be the supplement of vivo allergic reactions and have good clinical value.
- Subjects :
- Anaphylaxis blood
Anaphylaxis metabolism
Animals
Basophil Degranulation Test methods
Basophils metabolism
Biomarkers analysis
Disease Models, Animal
Female
Flow Cytometry
Lung pathology
Male
Ovalbumin administration & dosage
Phosphoric Diester Hydrolases blood
Phosphoric Diester Hydrolases immunology
Pyrophosphatases immunology
Random Allocation
Rats
Rats, Wistar
Tetraspanin 30 metabolism
Anaphylaxis diagnosis
Basophils immunology
Lung metabolism
Pyrophosphatases blood
Tetraspanin 30 blood
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1004-5619
- Volume :
- 28
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Fa yi xue za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 22812215