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[Affine sorbent with typtophil-threonil-tirosine for binding immunoglobulin G: sorption characteristics and aspects of practical application].

Authors :
Levashov PA
Ovchinnikova ED
Afanas'eva MI
Frid DA
Al'muko AA
Bespalova ZhD
Adamova IIu
Afanas'eva OI
PokrovskiÄ­ SN
Source :
Bioorganicheskaia khimiia [Bioorg Khim] 2012 Jan-Feb; Vol. 38 (1), pp. 58-63.
Publication Year :
2012

Abstract

New chromatographic material based on tryptophil-threonil-tirosine was prepared. This sorbent effectively binds human, sheep, goat and cow immunoglobulins G. New sorbent shows high selectivity for removing immunoglobulins from blood plasma. Effective sorption capacity is 15-25 mg of immunoglobulin G per ml of matrix. Optimal method of covalent attachment ligand to polysaccharide matrix allows achieving high stability of the sorbents in terms of use and storage. This sorbent can be used in medicine and biotechnology.

Details

Language :
Russian
ISSN :
0132-3423
Volume :
38
Issue :
1
Database :
MEDLINE
Journal :
Bioorganicheskaia khimiia
Publication Type :
Academic Journal
Accession number :
22792706
Full Text :
https://doi.org/10.1134/s1068162012010141