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Immunophenotyping and extracellular matrix remodeling in pulmonary and extrapulmonary sarcoidosis.
- Source :
-
Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia [J Bras Pneumol] 2012 May-Jun; Vol. 38 (3), pp. 321-30. - Publication Year :
- 2012
-
Abstract
- Objective: To investigate the significance of cellular immune markers, as well as that of collagen and elastic components of the extracellular matrix, within granulomatous structures in biopsies of patients with pulmonary or extrapulmonary sarcoidosis.<br />Methods: We carried out qualitative and quantitative evaluations of inflammatory cells, collagen fibers, and elastic fibers in granulomatous structures in surgical biopsies of 40 patients with pulmonary and extrapulmonary sarcoidosis using histomorphometry, immunohistochemistry, picrosirius red staining, and Weigert's resorcin-fuchsin staining.<br />Results: The extrapulmonary tissue biopsies presented significantly higher densities of lymphocytes, macrophages, and neutrophils than did the lung tissue biopsies. Pulmonary granulomas showed a significantly higher number of collagen fibers and a lower density of elastic fibers than did extrapulmonary granulomas. The amount of macrophages in the lung samples correlated with FVC (p < 0.05), whereas the amount of CD3+, CD4+, and CD8+ lymphocytes correlated with the FEV1/FVC ratio and VC. There were inverse correlations between TLC and the CD1a+ cell count (p < 0.05), as well as between DLCO and collagen/elastic fiber density (r = -0.90; p = 0.04).<br />Conclusions: Immunophenotyping and remodeling both showed differences between pulmonary and extrapulmonary sarcoidosis in terms of the characteristics of the biopsy samples. These differences correlated with the clinical and spirometric data obtained for the patients, suggesting that two different pathways are involved in the mechanism of antigen clearance, which was more effective in the lungs and lymph nodes.
- Subjects :
- Adult
Aged
Aged, 80 and over
Analysis of Variance
Biopsy
Collagen immunology
Elastic Tissue immunology
Elastic Tissue pathology
Extracellular Matrix pathology
Female
Granuloma, Respiratory Tract immunology
Granuloma, Respiratory Tract pathology
Humans
Lung immunology
Lung pathology
Lymph Nodes immunology
Lymph Nodes pathology
Lymphocytes immunology
Macrophages, Alveolar immunology
Male
Middle Aged
Sarcoidosis pathology
Sarcoidosis, Pulmonary immunology
Sarcoidosis, Pulmonary pathology
Extracellular Matrix immunology
Immunity, Cellular
Immunophenotyping methods
Sarcoidosis immunology
Subjects
Details
- Language :
- English; Portuguese
- ISSN :
- 1806-3756
- Volume :
- 38
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia
- Publication Type :
- Academic Journal
- Accession number :
- 22782602
- Full Text :
- https://doi.org/10.1590/s1806-37132012000300007