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[Effect of GPER on the activation of PI3K/Akt induced by 17β-estradiol in endometrial carcinoma cells].
- Source :
-
Zhonghua fu chan ke za zhi [Zhonghua Fu Chan Ke Za Zhi] 2012 Apr; Vol. 47 (4), pp. 292-6. - Publication Year :
- 2012
-
Abstract
- Objective: To investigate the expression of G protein-coupled ER (GPER) and ER in the activation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) induced by 17β-estradiol (17β-E(2))in endometrial carcinoma cells, Ishikawa and HEC-1A.<br />Methods: Expressions of GPER, ERα and ERβ protein in Ishikawa and HEC-1A cells were detected by immunohistochemical SP method. Levels of GPER, ERα and ERβ were examined by western blot in Ishikawa and HEC-1A cells after treated with 1×10(-6) mol/L 17β-E(2) at different time (0, 15, 30, 60, 120 minutes).<br />Results: GPER was positive expressed in Ishikawa and HEC-1A cells. ERα and ERβ were both positive expressed in Ishikawa cells. While, ERα was weakly expressed and ERβ was almost negatively expressed in HEC-1A cells. Western blot analysis showed that 1×10(-6) mol/L 17β-E(2) treatment, the Ishikawa and HEC-1A cells GPER protein level for 15 minutes markedly increased (P < 0.05), which Ishikawa 30 minutes, when cells reached the highest level (0.192 ± 0.004), HEC-1A cells for 15 minutes and reached the highest level (0.184 ± 0.006); Ishikawa and HEC-1A cells, Akt, activation of 15 minutes from the treatment start was significantly increased (P < 0.05), which Ishikawa cells for 30 minutes and reached the highest level (0.666 ± 0.021), HEC-1A cells for 15 minutes and reached maximum (0.788 ± 0.035); Ishikawa and HEC-1A cells, ERα and ERβ protein expression did not change significantly (P > 0.05).<br />Conclusion: GPER likely involved in non-nuclear activation of PI3K/Akt signaling pathways in endometrial carcinoma cells, Ishikawa and HEC-1A.
- Subjects :
- Cell Line, Tumor
Cell Nucleus metabolism
Endometrial Neoplasms pathology
Enzyme Activation drug effects
Estrogen Receptor alpha metabolism
Estrogen Receptor beta metabolism
Female
Humans
Immunohistochemistry
Signal Transduction
Endometrial Neoplasms metabolism
Estradiol pharmacology
Phosphatidylinositol 3-Kinase metabolism
Proto-Oncogene Proteins c-akt metabolism
Receptors, Estrogen metabolism
Receptors, G-Protein-Coupled metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0529-567X
- Volume :
- 47
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Zhonghua fu chan ke za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 22781117