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The host endocytic pathway is essential for Plasmodium berghei late liver stage development.
- Source :
-
Traffic (Copenhagen, Denmark) [Traffic] 2012 Oct; Vol. 13 (10), pp. 1351-63. Date of Electronic Publication: 2012 Aug 03. - Publication Year :
- 2012
-
Abstract
- The obligate intracellular liver stage of the Plasmodium parasite represents a bottleneck in the parasite life cycle and remains a promising target for therapeutic intervention. During this stage, parasites undergo dramatic morphological changes and achieve one of the fastest replication rates among eukaryotic species. Nevertheless, relatively little is known about the parasite interactions with the host hepatocyte. Using immunofluorescence, live cell imaging and electron microscopy, we show that Plasmodium berghei parasites are surrounded by vesicles from the host late endocytic pathway. We found that these vesicles are acidic and contain the membrane markers Rab7a, CD63 and LAMP1. When host cell vesicle acidification was disrupted using ammonium chloride or Concanamycin A during the late liver stage of infection, parasite survival was not affected, but schizont size was significantly decreased. Furthermore, when the host cell endocytic pathway was loaded with BSA-gold, gold particles were found within the parasite cytoplasm, showing the transport of material from the host endocytic pathway toward the parasite interior. These observations reveal a novel Plasmodium-host interaction and suggest that vesicles from the host endolysosomal pathway could represent an important source of nutrients exploited by the fast-growing late liver stage parasites.<br /> (© 2012 John Wiley & Sons A/S.)
- Subjects :
- Animals
Cytoplasmic Vesicles chemistry
Female
Lysosomal Membrane Proteins analysis
Mice
Mice, Inbred C57BL
Schizonts growth & development
Tetraspanin 30 analysis
Tumor Cells, Cultured
rab GTP-Binding Proteins analysis
rab7 GTP-Binding Proteins
Endocytosis
Hepatocytes metabolism
Hepatocytes parasitology
Host-Parasite Interactions
Plasmodium berghei growth & development
Plasmodium berghei metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0854
- Volume :
- 13
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Traffic (Copenhagen, Denmark)
- Publication Type :
- Academic Journal
- Accession number :
- 22780869
- Full Text :
- https://doi.org/10.1111/j.1600-0854.2012.01398.x