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Carnosine prevents necrotic and apoptotic death of rat thymocytes via ouabain-sensitive Na/K-ATPase.
- Source :
-
Cell biochemistry and function [Cell Biochem Funct] 2013 Jan; Vol. 31 (1), pp. 30-5. Date of Electronic Publication: 2012 Jul 04. - Publication Year :
- 2013
-
Abstract
- It is known that ouabain, a selective inhibitor of Na/K-ATPase, not only can cause the activation of signal cascades, which regulate the cell viability, but also can cause the accumulation of free radicals, which can evoke the oxidative stress. We have shown that the nanomolar concentrations of ouabain result in the temporary increase in the level of intracellular free radicals, but the millimolar concentration of ouabain induces a stable intracellular accumulation of free radicals in rat thymocytes. The increasing level of free radicals resulting from both low and high concentrations of ouabain can be attenuated by the antioxidant, carnosine. Moreover, the long-term incubation with ouabain leads to the cell death by necrosis and apoptosis. Ouabain-mediated apoptosis and necrosis were also abolished by carnosine.<br /> (Copyright © 2012 John Wiley & Sons, Ltd.)
- Subjects :
- Animals
Apoptosis drug effects
Calcium Signaling drug effects
Culture Media pharmacology
Dose-Response Relationship, Drug
Enzyme Activation drug effects
Free Radicals metabolism
Necrosis
Nitric Oxide Synthase metabolism
Nitroarginine pharmacology
Ouabain pharmacology
Protein Isoforms drug effects
Rats
Rats, Inbred WKY
Signal Transduction drug effects
Thymocytes cytology
Thymocytes enzymology
Carnosine pharmacology
Ouabain antagonists & inhibitors
Sodium-Potassium-Exchanging ATPase drug effects
Thymocytes drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0844
- Volume :
- 31
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell biochemistry and function
- Publication Type :
- Academic Journal
- Accession number :
- 22763713
- Full Text :
- https://doi.org/10.1002/cbf.2856