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Restoring specific lactobacilli levels decreases inflammation and muscle atrophy markers in an acute leukemia mouse model.
- Source :
-
PloS one [PLoS One] 2012; Vol. 7 (6), pp. e37971. Date of Electronic Publication: 2012 Jun 27. - Publication Year :
- 2012
-
Abstract
- The gut microbiota has recently been proposed as a novel component in the regulation of host homeostasis and immunity. We have assessed for the first time the role of the gut microbiota in a mouse model of leukemia (transplantation of BaF3 cells containing ectopic expression of Bcr-Abl), characterized at the final stage by a loss of fat mass, muscle atrophy, anorexia and inflammation. The gut microbial 16S rDNA analysis, using PCR-Denaturating Gradient Gel Electrophoresis and quantitative PCR, reveals a dysbiosis and a selective modulation of Lactobacillus spp. (decrease of L. reuteri and L. johnsonii/gasseri in favor of L. murinus/animalis) in the BaF3 mice compared to the controls. The restoration of Lactobacillus species by oral supplementation with L. reuteri 100-23 and L. gasseri 311476 reduced the expression of atrophy markers (Atrogin-1, MuRF1, LC3, Cathepsin L) in the gastrocnemius and in the tibialis, a phenomenon correlated with a decrease of inflammatory cytokines (interleukin-6, monocyte chemoattractant protein-1, interleukin-4, granulocyte colony-stimulating factor, quantified by multiplex immuno-assay). These positive effects are strain- and/or species-specific since L. acidophilus NCFM supplementation does not impact on muscle atrophy markers and systemic inflammation. Altogether, these results suggest that the gut microbiota could constitute a novel therapeutic target in the management of leukemia-associated inflammation and related disorders in the muscle.
- Subjects :
- Acute Disease
Animals
Cells, Cultured
Dietary Supplements
Female
Fusion Proteins, bcr-abl genetics
Gastrointestinal Tract microbiology
Inflammation etiology
Leukemia, Experimental genetics
Leukemia, Experimental pathology
Liver Neoplasms metabolism
Liver Neoplasms microbiology
Liver Neoplasms pathology
Metagenome
Mice
Mice, Inbred BALB C
Muscular Atrophy etiology
Precursor Cells, B-Lymphoid transplantation
Splenic Neoplasms metabolism
Splenic Neoplasms microbiology
Splenic Neoplasms pathology
Disease Models, Animal
Inflammation prevention & control
Inflammation Mediators metabolism
Lactobacillus physiology
Leukemia, Experimental complications
Muscular Atrophy prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 7
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22761662
- Full Text :
- https://doi.org/10.1371/journal.pone.0037971