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Pre-administration of L-tryptophan improved ADR-induced early renal failure in mice.
- Source :
-
Life sciences [Life Sci] 2012 Aug 21; Vol. 91 (3-4), pp. 100-6. Date of Electronic Publication: 2012 Jun 28. - Publication Year :
- 2012
-
Abstract
- Aims: The aim of this study was to determine the localization of the rate-limiting enzyme indoleamine 2, 3-dioxygenase (IDO) and its metabolite in mice kidneys after adriamycin (ADR)-induced renal failure. We also examined the effect of L-tryptophan (Trp) administration on this model.<br />Main Methods: BALB/c mice were treated with 15 mg/kg ADR to induce renal failure. The change of IDO and L-kynurenine (Kyn) following ADR-induced renal failure was examined by immunostaining combined with hematoxylin and eosin (HE) and Periodic acid-Schiff (PAS) staining for morphological analysis, and the concentration of L-Kyn was measured by HPLC. The effect of L-Trp administration on ADR-induced renal failure was also investigated.<br />Key Findings: Time-dependent increase of IDO immunostaining was observed in tubular cells from Day 4 after ADR injection. It was found that mice fed with L-Trp had less chance of renal failure at four days after ADR injection than mice untreated with L-Trp, but not at seven days. Further, L-Trp treatment significantly suppressed an increased level of TNF-alpha mRNA, but not of TGF-beta and IL1-beta after renal injury.<br />Significance: Local IDO expression in tubules was induced markedly after ADR- induced renal failure. L-Trp administration can be effective in suppressing ADR-induced renal failure at an early stage.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Chromatography, High Pressure Liquid methods
Down-Regulation
Doxorubicin adverse effects
Eosine Yellowish-(YS) pharmacology
Hematoxylin pharmacology
Kidney metabolism
Kynurenine pharmacology
Male
Mice
Mice, Inbred BALB C
Periodic Acid pharmacology
RNA, Messenger metabolism
Schiff Bases pharmacology
Time Factors
Indoleamine-Pyrrole 2,3,-Dioxygenase pharmacology
Renal Insufficiency drug therapy
Tryptophan administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0631
- Volume :
- 91
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 22749866
- Full Text :
- https://doi.org/10.1016/j.lfs.2012.06.018