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Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range.
- Source :
-
Molecular bioSystems [Mol Biosyst] 2012 Oct; Vol. 8 (10), pp. 2531-4. - Publication Year :
- 2012
-
Abstract
- Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins.
- Subjects :
- Carbocyanines
Color
Escherichia coli genetics
Fluorescence Resonance Energy Transfer
Fluorescent Dyes
Humans
Hydrazines
Nuclear Pore Complex Proteins genetics
Organic Chemicals
Protein Conformation
Protein Structure, Tertiary
Recombinant Proteins chemistry
Recombinant Proteins genetics
Saccharomyces cerevisiae chemistry
Saccharomyces cerevisiae Proteins genetics
Spectrometry, Fluorescence
Molecular Imaging methods
Nuclear Pore Complex Proteins chemistry
Saccharomyces cerevisiae Proteins chemistry
Staining and Labeling methods
Subjects
Details
- Language :
- English
- ISSN :
- 1742-2051
- Volume :
- 8
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Molecular bioSystems
- Publication Type :
- Academic Journal
- Accession number :
- 22739670
- Full Text :
- https://doi.org/10.1039/c2mb25135c