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[Analysis of an acellular pigskin based nerve scaffold].
- Source :
-
Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2012 Mar; Vol. 28 (3), pp. 349-57. - Publication Year :
- 2012
-
Abstract
- A scaffold fabricated with lysine/nerve growth factor (NGF)/poly (lactic acid coglycolic acid) copolymer (PLGA) and acellular pigskin was evaluated in vitro as a potential artificial nerve scaffold. Properties of the scaffold such as microstructure, mechanical property, degradation behavior in PBS and water, Schwann cell adhesion property, and release of NGF were investigated. Results showed PLGA had permeated into the porous structure of acellular pigskin; its breaking strength was 8.308 MPa, breaking extensibility was 38.98%, elastic modulus was 97.27 MPa. The porosities of the scaffold ranged from 68.3% to 81.2% with densities from 0.62 g/cm3 to 0.68 g/cm3. At 4 weeks of degradation in vitro, maximum mass loss ratio was 43.3%. The release of NGF could still be detected on the 30th day, and its accumulative release rate was 38%. Lysine added into the scaffold neutralized the acidoid preventing degradation of PLGA to maintain a solution pH value. Schwann cells had grown across the scaffold after co-cultivation for 15 days. These in vitro properties of the pigskin based composite might indicate its potentiality to be an artificial nerve scaffold.
- Subjects :
- Animals
Biocompatible Materials
Guided Tissue Regeneration
Lysine pharmacology
Nerve Growth Factors chemistry
Polylactic Acid-Polyglycolic Acid Copolymer
Swine
Tissue Engineering
Acellular Dermis
Lactic Acid pharmacology
Nerve Growth Factors pharmacology
Nerve Regeneration
Polyglycolic Acid pharmacology
Tissue Scaffolds
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-3061
- Volume :
- 28
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 22712393