Back to Search
Start Over
Molecular analysis of hepatitis B virus associated with vaccine failure in infants and mothers: a case-control study in Thailand.
- Source :
-
Journal of medical virology [J Med Virol] 2012 Aug; Vol. 84 (8), pp. 1177-85. - Publication Year :
- 2012
-
Abstract
- Perinatal transmission of hepatitis B virus (HBV) has been controlled incompletely despite adequate immunoprophylaxis in infants. The aim of this study was to characterize virological factors of HBV associated with vaccine failure in Thailand. Sera of 14 infected infants (13 HBeAg-positive and one HBeAg-negative) with vaccine failure and their respective mothers (group M1) were tested quantitatively for HBV DNA by real-time PCR, HBV genotypes and mutations were characterized by direct sequencing. Sera collected from 15 HBeAg-positive (group M2) and 15 HBeAg-negative (group M3) mothers whose infants had been vaccinated successfully served as controls. The results showed that group M1 and group M2 mothers had equal titers of HBV DNA but higher titers than group M3. All infected infants and their respective mothers had the same HBeAg status and HBV genotypes. DNA analysis in a pair of HBeAg-negative infant and mother revealed that both were infected with an HBV precore mutant (G1896A). Escape mutants in the "a" determinant region (residues 144 and 145) were detected in two (14%) infected infants. The prevalence of BCP mutations/deletions in groups M2 and M3 was higher significantly than in group M1 (Pā=ā0.022 and Pā<ā0.001, respectively). In conclusion, instead of the HBeAg status, a high titer of HBV DNA in mothers was the major contributor to perinatal transmission of HBV. Escape mutants might be associated with vaccine failure in some infants. BCP mutations/deletions in mothers might contribute to the prevention of mother-to-infant transmission of HBV.<br /> (Copyright © 2012 Wiley Periodicals, Inc.)
- Subjects :
- Adult
Case-Control Studies
DNA, Viral analysis
DNA, Viral blood
Female
Genotype
Hepatitis B Vaccines administration & dosage
Hepatitis B e Antigens blood
Hepatitis B e Antigens genetics
Hepatitis B virus immunology
Hepatitis B, Chronic virology
Humans
Infant
Mutation
Pregnancy
Pregnancy Complications, Infectious virology
Thailand
Treatment Failure
Young Adult
Hepatitis B Vaccines therapeutic use
Hepatitis B virus genetics
Hepatitis B, Chronic prevention & control
Hepatitis B, Chronic transmission
Infectious Disease Transmission, Vertical
Pregnancy Complications, Infectious prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1096-9071
- Volume :
- 84
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of medical virology
- Publication Type :
- Academic Journal
- Accession number :
- 22711345
- Full Text :
- https://doi.org/10.1002/jmv.23260