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TGF-β affects enterocyte turnover in correlation with TGF-β receptor expression after massive small bowel resection.
- Source :
-
Journal of pediatric gastroenterology and nutrition [J Pediatr Gastroenterol Nutr] 2012 Dec; Vol. 55 (6), pp. 721-7. - Publication Year :
- 2012
-
Abstract
- Objectives: In the present study, we evaluated the effect of transforming growth factor-beta 2 (TGF-β2)-enriched diet on enterocyte turnover and correlated it with TGF-β2 receptor expression along the villus-crypt axis in a rat model of short bowel syndrome (SBS).<br />Methods: CaCo-2 cells were incubated with increasing concentrations of TGF-β2. Alamar Blue reduction test was used for investigation of cell viability and evaluation of cell apoptosis was assessed by flow cytometry. Male rats were divided into 4 groups: Sham rats underwent bowel transection, Sham TGF-β rats were treated with diet enriched with TGF-β2, SBS rats underwent a 75% bowel resection, and SBS TGF-β rats were fed a diet enriched with TGF-β2 after bowel resection. Parameters of intestinal adaptation, enterocyte proliferation and apoptosis were determined at sacrifice. TGF-β2r expression in villus tips, lateral villi and crypts was assessed by immunohistochemistry. The effect of TGF-β2 on enterocyte turnover for each compartment was evaluated in correlation with TGF-β2r expression.<br />Results: Incubation of CaCo-2 cells with TGF-β2 resulted in a significant decrease in cell viability and increased cell apoptosis. TGF-β2r expression in crypts increased in SBS rats (vs sham) and was accompanied by decreased cell proliferation and increased cell apoptosis following TGF-β2 administration. A significant decrease in TGF-β2r expression at villous tips in SBS rats was accompanied by a decreased cell apoptosis in this compartment following exposure to TGF-β2-enriched diet.<br />Conclusions: In a rat model of SBS, the inhibiting effect of TGF-β2 on enterocyte turnover correlates with TGF-β2 receptor expression along the villus-crypt axis.
- Subjects :
- Animals
Caco-2 Cells
Cell Survival drug effects
Enterocytes metabolism
Humans
Intestine, Small metabolism
Intestine, Small surgery
Male
Rats
Short Bowel Syndrome surgery
Transforming Growth Factor beta2 metabolism
Apoptosis drug effects
Cell Proliferation drug effects
Enterocytes drug effects
Intestine, Small drug effects
Receptors, Transforming Growth Factor beta metabolism
Short Bowel Syndrome metabolism
Transforming Growth Factor beta2 pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1536-4801
- Volume :
- 55
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of pediatric gastroenterology and nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 22711000
- Full Text :
- https://doi.org/10.1097/MPG.0b013e318263ec18