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Prevalence of a novel division-level bacterial lineage in Lake Dhanmondi, Dhaka, Bangladesh, as revealed by deep sequencing of 16S rRNA gene amplicons.
- Source :
-
Current microbiology [Curr Microbiol] 2012 Oct; Vol. 65 (4), pp. 356-60. Date of Electronic Publication: 2012 Jun 17. - Publication Year :
- 2012
-
Abstract
- A culture-independent study of the bacterial diversity in Lake Dhanmondi, located in the central region of Dhaka city, Bangladesh, was carried out using deep sequence analysis of 16S rRNA gene PCR amplicons. The results revealed the presence of a group of bacteria, termed LD11, phylogenetically unrelated to any previously cultivated bacteria at the phylum level. LD11 sequences comprised about 1.7 % of the total sequence reads after quality assessment. LD11 appears to constitute a novel division with a deep evolutionary lineage apparently branching between the Chloroflexi and Thermi-Deinococci phyla. Sequence similarity with molecular data from freshwater environments indicates that LD11 represents a widespread and novel clade of freshwater bacteria for which no cultivated representatives are yet available.
- Subjects :
- Bangladesh
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
High-Throughput Nucleotide Sequencing
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S genetics
Bacteria classification
Bacteria genetics
Biodiversity
Fresh Water microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0991
- Volume :
- 65
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Current microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 22706799
- Full Text :
- https://doi.org/10.1007/s00284-012-0165-9