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Nicotianamine functions in the Phloem-based transport of iron to sink organs, in pollen development and pollen tube growth in Arabidopsis.
- Source :
-
The Plant cell [Plant Cell] 2012 Jun; Vol. 24 (6), pp. 2380-400. Date of Electronic Publication: 2012 Jun 15. - Publication Year :
- 2012
-
Abstract
- The metal chelator nicotianamine promotes the bioavailability of Fe and reduces cellular Fe toxicity. For breeding Fe-efficient crops, we need to explore the fundamental impact of nicotianamine on plant development and physiology. The quadruple nas4x-2 mutant of Arabidopsis thaliana cannot synthesize any nicotianamine, shows strong leaf chlorosis, and is sterile. To date, these phenotypes have not been fully explained. Here, we show that sink organs of this mutant were Fe deficient, while aged leaves were Fe sufficient. Upper organs were also Zn deficient. We demonstrate that transport of Fe to aged leaves relied on citrate, which partially complemented the loss of nicotianamine. In the absence of nicotianamine, Fe accumulated in the phloem. Our results show that rather than enabling the long-distance movement of Fe in the phloem (as is the case for Zn), nicotianamine facilitates the transport of Fe from the phloem to sink organs. We delimit nicotianamine function in plant reproductive biology and demonstrate that nicotianamine acts in pollen development in anthers and pollen tube passage in the carpels. Since Fe and Zn both enhance pollen germination, a lack of either metal may contribute to the reproductive defect. Our study sheds light on the physiological functions of nicotianamine.
- Subjects :
- Arabidopsis genetics
Arabidopsis growth & development
Azetidinecarboxylic Acid metabolism
Biological Transport
Citrates metabolism
Flowers metabolism
Gene Expression Regulation, Plant
Mutation
Phloem metabolism
Plant Development
Plant Leaves genetics
Plant Leaves metabolism
Plant Roots metabolism
Pollen genetics
Pollen metabolism
Pollen Tube growth & development
Pollen Tube metabolism
Zinc metabolism
Arabidopsis metabolism
Azetidinecarboxylic Acid analogs & derivatives
Iron metabolism
Pollen growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1532-298X
- Volume :
- 24
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 22706286
- Full Text :
- https://doi.org/10.1105/tpc.112.099077