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Optimization of liquid fermentation of microbial consortium WSD-5 followed by saccharification and acidification of wheat straw.
- Source :
-
Bioresource technology [Bioresour Technol] 2012 Aug; Vol. 118, pp. 141-9. Date of Electronic Publication: 2012 May 17. - Publication Year :
- 2012
-
Abstract
- The microbial consortium WSD-5 is composed of bacteria and fungi, and the cooperation and symbiosis of the contained microbes enhance the degradation ability of WSD-5. Experiment results showed that the highest cellulase and hemicellulase were obtained when ventilation volume was 4 L/min, stirring rate was 0 rpm, and substrate loading rate was 3%. After 6 days of cultivation, a 67.60% loss in wheat straw dry weight was observed. The crude enzyme secreted from WSD-5 after optimization was evaluated by experiments of saccharification and acidification. The maximum concentration of reducing sugars was 3254 mg/L after 48 h saccharification. The concentration of sCOD peaked on day 2 with a value of 4345 mg/L during acidification, and the biogas yield and methane yield were 22.3% and 32.3% higher than un-acidified samples. This study is the first attempt to explore both the saccharification and the acidification ability of crude enzymes secreted by microbial consortium.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- Anaerobiosis
Biodegradation, Environmental
Biodiversity
Denaturing Gradient Gel Electrophoresis
Enzymes metabolism
Polymerase Chain Reaction
RNA, Ribosomal genetics
Substrate Specificity
Acids chemistry
Biotechnology methods
Carbohydrate Metabolism
Fermentation physiology
Microbial Consortia genetics
Triticum chemistry
Waste Products analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 118
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 22705517
- Full Text :
- https://doi.org/10.1016/j.biortech.2012.05.025