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The role of SIRT1 on angiogenic and odontogenic potential in human dental pulp cells.
- Source :
-
Journal of endodontics [J Endod] 2012 Jul; Vol. 38 (7), pp. 899-906. Date of Electronic Publication: 2012 May 19. - Publication Year :
- 2012
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Abstract
- Introduction: Although several biological roles of SIRT1 have been reported, the expression and role of SIRT1 in human dental pulp cells (HDPCs) remain unknown. To identify the role of SIRT1 in HDPCS, we measured SIRT1 messenger RNA and protein levels during the odontoblastic differentiation of HDPCs. Additionally, we investigated the effects of SIRT1 overexpression and knockdown on the differentiation of HDPCs.<br />Methods: The expression of markers for odontoblastic differentiation and angiogenesis was analyzed by alizarin red staining, reverse-transcriptase polymerase chain reaction, and Western blot analysis.<br />Results: A transient increase in SIRT1 gene expression occurred in early odontogenesis, which peaked at 1 day and decreased thereafter. SIRT1 induction by resveratrol and by infection with adenovirus-SIRT1 (Ad-SIRT1), a SIRT1-expressing adenoviral vector, increased mineralization nodules and up-regulated messenger RNA expression of odontoblastic markers (ie, alkaline phosphatase, osteocalcin, dentin matrix protein-1, and dentin sialophosphoprotein) as well as angiogenic markers (ie, vascular endothelial growth factor, endothelial cell adhesion molecules such as vascular endothelial cadherin and platelet endothelial cell adhesion molecule 1, and fibroblast growth factor-2). In contrast, the inhibition of SIRT1 expression by sirtinol and SIRT1 small interfering RNAs decreased odontoblastic differentiation and down-regulated angiogenic factors.<br />Conclusions: SIRT1 gene activation may provide therapeutic effects in pulp regeneration and dentin tissue engineering.<br /> (Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Alkaline Phosphatase biosynthesis
Analysis of Variance
Antigens, CD biosynthesis
Cadherins biosynthesis
Cell Differentiation physiology
Cell Line, Transformed
Dental Pulp blood supply
Dental Pulp cytology
Extracellular Matrix Proteins biosynthesis
Fibroblast Growth Factor 2 biosynthesis
Gene Expression
Gene Knockdown Techniques
Humans
Odontoblasts cytology
Odontoblasts physiology
Osteocalcin biosynthesis
Phosphoproteins biosynthesis
Platelet Endothelial Cell Adhesion Molecule-1 biosynthesis
RNA, Messenger analysis
Regeneration genetics
Sialoglycoproteins biosynthesis
Up-Regulation
Vascular Endothelial Growth Factor A biosynthesis
Dental Pulp physiology
Neovascularization, Physiologic genetics
Odontoblasts metabolism
Odontogenesis genetics
Sirtuin 1 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3554
- Volume :
- 38
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of endodontics
- Publication Type :
- Academic Journal
- Accession number :
- 22703651
- Full Text :
- https://doi.org/10.1016/j.joen.2012.04.006