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Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma.
- Source :
-
International journal of oncology [Int J Oncol] 2012 Sep; Vol. 41 (3), pp. 1061-7. Date of Electronic Publication: 2012 Jun 12. - Publication Year :
- 2012
-
Abstract
- Hepatocellular carcinoma (HCC) is a type of cancer with a very poor prognosis. Although α-fetoprotein (AFP) is the most effective marker available to detect HCC, the sensitivity and specificity are not optimal. Therefore, there is a need for the development of more sensitive and specific methods that can supplement AFP in the early detection of this cancer. In this study, autoantibody responses to glucose-regulated protein 78 (GRP78) were evaluated by enzyme-linked immunosorbent assay (ELISA), western blotting and indirect immunofluorescence assay in sera from patients with HCC, liver cirrhosis (LC) and chronic hepatitis (CH), as well as from normal human individuals. Immunohistochemistry (IHC) with tissue array slides was also preformed to analyze protein expression profiles of GRP78 in HCC and control tissues. The prevalence of autoantibodies against GRP78 was 35.5% (27/76) in HCC, which was significantly higher than that in LC, CH and normal human sera (NHS; P<0.01). The average titer of autoantibodies against GRP78 in HCC sera was higher compared to that in LC, CH and NHS(P<0.01). When both autoantibodies against GRP78 and AFP were used simultaneously as diagnostic markers, sensitivity reached 71.4%. Our data indicate that anti-GRP78 autoantibodies may be potential diagnostic markers for HCC, especially in conjunction with AFP.
- Subjects :
- Adult
Aged
Autoantibodies immunology
Biomarkers, Tumor blood
Blotting, Western
Carcinoma, Hepatocellular immunology
Cell Line, Tumor
Endoplasmic Reticulum Chaperone BiP
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique, Indirect
Heat-Shock Proteins metabolism
Hepatitis, Chronic immunology
Humans
Liver Cirrhosis immunology
Liver Neoplasms immunology
Male
Middle Aged
Autoantibodies blood
Carcinoma, Hepatocellular diagnosis
Heat-Shock Proteins immunology
Liver Neoplasms diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2423
- Volume :
- 41
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of oncology
- Publication Type :
- Academic Journal
- Accession number :
- 22692946
- Full Text :
- https://doi.org/10.3892/ijo.2012.1515