Back to Search
Start Over
Improved method for the isolation, characterization and examination of neuromuscular and toxic properties of selected polypeptide fractions from the crude venom of the Taiwan cobra Naja naja atra.
- Source :
-
Toxicon : official journal of the International Society on Toxinology [Toxicon] 2012 Sep 15; Vol. 60 (4), pp. 623-31. Date of Electronic Publication: 2012 Jun 04. - Publication Year :
- 2012
-
Abstract
- An improved chromatographic method was developed to isolate and purify polypeptides and proteins from the crude venom of the Taiwan cobra Naja naja atra. The procedure devised is simple, easy to reproduce, and enables large scale isolation of almost all polypeptides and proteins in this cobra venom. Six pure polypeptide fractions of the venom were isolated and characterized using gel filtration on Sephadex G50 (medium), ion exchange chromatography on SP-Sephadex C25, desalting on Sephadex G25 (fine) and preparative HPLC on a RPC 18 column. The neuromuscular activity of these fractions was tested on the chick biventer cervicis nerve-muscle preparation and their toxicity (LD(50)) was determined after i.v. administration in mice. Their antinociceptive activity was tested in the mouse abdominal test by i.v. application. Two of these polypeptide samples had major physiological effects: one acted as a cardiotoxin causing reversible myocardial contractures with no effect on muscle twitches elicited by nerve stimulation (NS); another was a neurotoxin that blocked muscle contractions in response to NS and exogenously added acetylcholine. The cardiotoxic fraction was identified as CTX I, a well-known cardiotoxin present in this venom, and the neurotoxin was identified as neurotoxin-α with an LD50 in mice of 0.075 mg/kg.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- Analgesics isolation & purification
Analgesics pharmacology
Animals
Chemical Fractionation instrumentation
Chemical Fractionation methods
Chickens
Cobra Cardiotoxin Proteins chemistry
Cobra Cardiotoxin Proteins toxicity
Cobra Neurotoxin Proteins chemistry
Cobra Neurotoxin Proteins toxicity
Elapid Venoms chemistry
Elapid Venoms toxicity
Elapidae physiology
Female
Heart drug effects
Lethal Dose 50
Male
Mice
Muscle Contraction drug effects
Muscle, Skeletal drug effects
Myocardial Contraction drug effects
Neuromuscular Junction drug effects
Neuromuscular Junction physiopathology
Pain chemically induced
Pain drug therapy
Pain Threshold drug effects
Peptides chemistry
Peptides toxicity
Rats
Chromatography methods
Cobra Cardiotoxin Proteins isolation & purification
Cobra Neurotoxin Proteins isolation & purification
Elapid Venoms metabolism
Peptides isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3150
- Volume :
- 60
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Toxicon : official journal of the International Society on Toxinology
- Publication Type :
- Academic Journal
- Accession number :
- 22677803
- Full Text :
- https://doi.org/10.1016/j.toxicon.2012.05.013