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Altered desialylated plasma N-glycan profile in patients with non-small cell lung carcinoma.
- Source :
-
Cancer biomarkers : section A of Disease markers [Cancer Biomark] 2011-2012; Vol. 10 (3-4), pp. 145-54. - Publication Year :
- 2011
-
Abstract
- Successful therapy of the non-small cell lung carcinoma (NSCLC) depends on its early detection, and non-invasive detection methods are preferred. As plasma proteins are modified by N-linked glycosylation, we tested the importance of the N-glycan profile in diagnosing and prognosticating NSCLC. We analysed desialylated plasma samples from 75 NSCLC patients, and 71 healthy individuals by the high-throughput DNA sequencer-based carbohydrate analytical profiling technique. We detected alterations in the levels of several N-glycans in NSCLC patients. Total α-1,6-core fucosylated biantennaries (NGA2F, NG1A2F, NA2F) and total bisecting α-1,6-core fucosylated biantennaries (NGA2FB, NA2FB) were reduced in NSCLC patients, whereas the branching α-1,3-fucosylated triantennary N-glycan (NA3FB) was increased. Best diagnostic accuracy was identified for NG1A2F. NSCLC patients with TNM stage I stage did not show further differences, but patients with higher stages did (TNM II to IV). Those patients additionally had a reduced level in the α-1,6-core fucosylated structure NA2F with parallel increase in the non-fucosylated structure NA2. In this regard, NSCLC patients with a relatively low amount of NA2 per NA2F had a better three-year survival than patients with high amount. NSCLC patients show an altered N-glycan profile of plasma proteins that may be regarded as a supportive tool for cancer diagnosis.
- Subjects :
- Aged
Carcinoma, Non-Small-Cell Lung diagnosis
Female
Glycomics methods
Humans
Lung Neoplasms diagnosis
Male
Middle Aged
N-Acetylneuraminic Acid chemistry
Neoplasm Staging
Polysaccharides chemistry
Prognosis
ROC Curve
Survival Analysis
Biomarkers, Tumor blood
Carcinoma, Non-Small-Cell Lung blood
Lung Neoplasms blood
Polysaccharides blood
Subjects
Details
- Language :
- English
- ISSN :
- 1875-8592
- Volume :
- 10
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Cancer biomarkers : section A of Disease markers
- Publication Type :
- Academic Journal
- Accession number :
- 22674300
- Full Text :
- https://doi.org/10.3233/CBM-2012-0239