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Two methods to easily obtain nucleotide sequences from AFLP loci of interest.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 888, pp. 91-108. - Publication Year :
- 2012
-
Abstract
- Genome scans based on anonymous Amplified Fragment Length Polymorphism (AFLP) markers scattered throughout the genome are becoming an increasingly popular approach to study the genetic basis of adaptation and speciation in natural populations. A shortcoming of this approach is that despite its efficiency to detect signatures of selection, it can hardly help pinpoint the specific genomic region(s), gene(s), or mutation(s) targeted by selection. Here, we present two methods to be undertaken after performing an AFLP-based genome scan to easily obtain the sequences of AFLP loci detected as outliers by population genomics approaches. The first one is based on the gel excision of the target AFLP fragment, after simplification of the AFLP fingerprint and separation of the fragments by migration. The second one is a combination of classical AFLP protocol and 454 pyrosequencing.
- Subjects :
- Adaptation, Physiological genetics
Animals
Bacteria
Electrophoresis, Polyacrylamide Gel
Fungi
Genetic Loci
Genetic Speciation
High-Throughput Nucleotide Sequencing
Plants
Polymorphism, Genetic
Selection, Genetic
Amplified Fragment Length Polymorphism Analysis methods
Base Sequence genetics
Genome
Genomics methods
Sequence Analysis, DNA methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 888
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 22665277
- Full Text :
- https://doi.org/10.1007/978-1-61779-870-2_6