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Preparation and characterization of a VEGF-Fc fusion protein matrix for enhancing HUVEC growth.
- Source :
-
Biotechnology letters [Biotechnol Lett] 2012 Sep; Vol. 34 (9), pp. 1765-71. Date of Electronic Publication: 2012 Jun 03. - Publication Year :
- 2012
-
Abstract
- To enhance vascularization of hydrophobic implants in vivo, a VEGF-Fc fusion protein consisting of vascular endothelial growth factor (VEGF) fused to the immunoglobulin G Fc domain was prepared as an artificial extracellular matrix (ECM). VEGF-Fc was stably immobilized on a polystyrene plate due to the hydrophobicity of the Fc domain, and significantly enhanced the adhesion of human umbilical vein endothelial cells (HUVECs). Additionally, the use of VEGF-Fc as an ECM markedly promoted the proliferation of HUVECs longer than 72 h and induced the reorganization of actin filaments into larger stress fibers within these cells. The VEGF-Fc fusion protein may be a promising artificial ECM for enhancing endothelial cell growth.
- Subjects :
- Cell Culture Techniques methods
Extracellular Matrix genetics
Extracellular Matrix metabolism
Humans
Hydrophobic and Hydrophilic Interactions
Immunoglobulin Fc Fragments genetics
Immunoglobulin Fc Fragments metabolism
Polystyrenes chemistry
Protein Binding
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Time Factors
Vascular Endothelial Growth Factor A genetics
Vascular Endothelial Growth Factor A metabolism
Cell Proliferation
Endothelial Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6776
- Volume :
- 34
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 22661013
- Full Text :
- https://doi.org/10.1007/s10529-012-0959-7