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Chitin-induced dimerization activates a plant immune receptor.
- Source :
-
Science (New York, N.Y.) [Science] 2012 Jun 01; Vol. 336 (6085), pp. 1160-4. - Publication Year :
- 2012
-
Abstract
- Pattern recognition receptors confer plant resistance to pathogen infection by recognizing the conserved pathogen-associated molecular patterns. The cell surface receptor chitin elicitor receptor kinase 1 of Arabidopsis (AtCERK1) directly binds chitin through its lysine motif (LysM)-containing ectodomain (AtCERK1-ECD) to activate immune responses. The crystal structure that we solved of an AtCERK1-ECD complexed with a chitin pentamer reveals that their interaction is primarily mediated by a LysM and three chitin residues. By acting as a bivalent ligand, a chitin octamer induces AtCERK1-ECD dimerization that is inhibited by shorter chitin oligomers. A mutation attenuating chitin-induced AtCERK1-ECD dimerization or formation of nonproductive AtCERK1 dimer by overexpression of AtCERK1-ECD compromises AtCERK1-mediated signaling in plant cells. Together, our data support the notion that chitin-induced AtCERK1 dimerization is critical for its activation.
- Subjects :
- Acetylglucosamine chemistry
Acetylglucosamine metabolism
Amino Acid Motifs
Amino Acid Sequence
Arabidopsis immunology
Arabidopsis Proteins genetics
Binding Sites
Chitin chemistry
Crystallography, X-Ray
Hydrogen Bonding
Ligands
Models, Molecular
Molecular Sequence Data
Mutant Proteins chemistry
Mutant Proteins metabolism
Phosphorylation
Plants, Genetically Modified
Protein Multimerization
Protein Serine-Threonine Kinases genetics
Protein Structure, Tertiary
Receptors, Pattern Recognition genetics
Signal Transduction
Arabidopsis metabolism
Arabidopsis Proteins chemistry
Arabidopsis Proteins metabolism
Chitin metabolism
Protein Serine-Threonine Kinases chemistry
Protein Serine-Threonine Kinases metabolism
Receptors, Pattern Recognition chemistry
Receptors, Pattern Recognition metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 336
- Issue :
- 6085
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 22654057
- Full Text :
- https://doi.org/10.1126/science.1218867