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High-resolution protein structure determination by serial femtosecond crystallography.
- Source :
-
Science (New York, N.Y.) [Science] 2012 Jul 20; Vol. 337 (6092), pp. 362-4. Date of Electronic Publication: 2012 May 31. - Publication Year :
- 2012
-
Abstract
- Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 337
- Issue :
- 6092
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 22653729
- Full Text :
- https://doi.org/10.1126/science.1217737