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Mannosylglycerate stabilizes staphylococcal nuclease with restriction of slow β-sheet motions.
- Source :
-
Protein science : a publication of the Protein Society [Protein Sci] 2012 Aug; Vol. 21 (8), pp. 1126-37. Date of Electronic Publication: 2012 Jun 15. - Publication Year :
- 2012
-
Abstract
- Mannosylglycerate is a compatible solute typical of thermophilic marine microorganisms that has a remarkable ability to protect proteins from thermal denaturation. This ionic solute appears to be a universal stabilizing agent, but the extent of protection depends on the specific protein examined. To understand how mannosylglycerate confers protection, we have been studying its influence on the internal motions of a hyperstable staphylococcal nuclease (SNase). Previously, we found a correlation between the magnitude of protein stabilization and the restriction of fast backbone motions. We now report the effect of mannosylglycerate on the fast motions of side-chains and on the slower unfolding motions of the protein. Side-chain motions were assessed by (13)CH(3) relaxation measurements and model-free analysis while slower unfolding motions were probed by H/D exchange measurements at increasing concentrations of urea. Side-chain motions were little affected by the presence of different concentrations of mannosylglycerate or even by the presence of urea (0.25M), and show no correlation with changes in the thermodynamic stability of SNase. Native hydrogen exchange experiments showed that, contrary to reports on other stabilizing solutes, mannosylglycerate restricts local motions in addition to the global motions of the protein. The protein unfolding/folding pathway remained undisturbed in the presence of mannosylglycerate but the solute showed a specific effect on the local motions of β-sheet residues. This work reinforces the link between solute-induced stabilization and restriction of protein motions at different timescales, and shows that the solute preferentially affects specific structural elements of SNase.<br /> (Copyright © 2012 The Protein Society.)
- Subjects :
- Mannose metabolism
Models, Molecular
Molecular Dynamics Simulation
Protein Denaturation
Protein Folding
Protein Stability
Protein Structure, Secondary
Staphylococcus aureus chemistry
Staphylococcus aureus metabolism
Thermodynamics
Excipients metabolism
Glyceric Acids metabolism
Mannose analogs & derivatives
Micrococcal Nuclease chemistry
Micrococcal Nuclease metabolism
Staphylococcus aureus enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1469-896X
- Volume :
- 21
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Protein science : a publication of the Protein Society
- Publication Type :
- Academic Journal
- Accession number :
- 22619184
- Full Text :
- https://doi.org/10.1002/pro.2100