The complex of cytochrome f and plastocyanin from Nostoc sp. PCC 7119 is highly dynamic.

Authors :: Scanu S
Förster J
Finiguerra MG
Shabestari MH
Huber M
Ubbink M
Source :: Chembiochem : a European journal of chemical biology [Chembiochem] 2012 Jun 18; Vol. 13 (9), pp. 1312-8. Date of Electronic Publication: 2012 May 22.
Publication Year :: 2012
Abstract: Cytochrome f (Cyt f) and plastocyanin (Pc) form a highly transient complex as part of the photosynthetic redox chain. The complex from Nostoc sp. PCC 7119 was studied by NMR relaxation spectroscopy with the aim of determining the orientation of Pc relative to Cyt f. Chemical-shift-perturbation analysis showed that the presence of spin labels on the surface of Cyt f does not significantly affect the binding of Pc. The paramagnetic relaxation enhancement results are not consistent with a single orientation of Pc, thus indicating that multiple orientations must occur and suggesting that an encounter state represents a large fraction of the complex.<br /> (Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: Cytochromes f chemistry
Mesylates metabolism
Models, Molecular
Nuclear Magnetic Resonance, Biomolecular
Plastocyanin chemistry
Protein Binding
Protein Conformation
Cytochromes f metabolism
Nostoc
Plastocyanin metabolism

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