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[The construction and expression of Saccharomyces albicans pACT1-GFP plasmids].
- Source :
-
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology [Hua Xi Kou Qiang Yi Xue Za Zhi] 2012 Apr; Vol. 30 (2), pp. 115-8. - Publication Year :
- 2012
-
Abstract
- Objective: To construct strains containing green fluorescent protein (GFP) to study gene regulation in Saccharomyces albicans cells during the infection process.<br />Methods: pACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy.<br />Results: 99% of Saccharomyces albicans cells containing pACT1-GFP fusion displayed significant fluorescence levels both in the yeast and hyphal compartments. The fluorescence intensity in two compartments had no obvious difference.<br />Conclusion: pACT1-GFP can be expressed stably in the yeast cells.
Details
- Language :
- Chinese
- ISSN :
- 1000-1182
- Volume :
- 30
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology
- Publication Type :
- Academic Journal
- Accession number :
- 22594223