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CD4+ T-cell immunity after pandemic influenza vaccination cross-reacts with seasonal antigens and functionally differs from active influenza infection.
- Source :
-
European journal of immunology [Eur J Immunol] 2012 Jul; Vol. 42 (7), pp. 1755-66. - Publication Year :
- 2012
-
Abstract
- Antigen-specific antibodies are well characterized after vaccination with pandemic H1N1 or seasonal influenza vaccines. However, knowledge on cellular immunity toward pandemic H1N1 after vaccination and infection and cross-reactivities toward seasonal antigens is limited. Nineteen individuals were vaccinated with the pandemic H1N1 vaccine. Among those, ten had been prevaccinated against seasonal influenza. CD4(+) T cells specific for pandemic H1N1 and for seasonal vaccine, and antibodies were monitored using flow cytometry and ELISA/neutralization assays, respectively. In addition, seven patients with acute pandemic influenza infection were analyzed. Pandemic H1N1 vaccination induced a strong 4.63-fold (IQR 4.16) increase in antigen-specific CD4(+) T cells that was more pronounced in individuals not prevaccinated with seasonal influenza (p = 0.01). T-cell levels toward seasonal vaccine concomitantly rose by 2.71-fold (IQR 2.26). Likewise, prevaccination with seasonal influenza induced a less pronounced increase in specific antibodies. Influenza-specific T cells in vaccinees had a Th1 phenotype mainly coexpressing IFN-γ and IL-2, whereas patients with active pandemic influenza showed a shift toward cells predominantly expressing IFN-γ. In conclusion, T cells toward seasonal influenza antigens cross-react with pandemic H1N1 antigens and affect induction of specific T cells after pandemic influenza vaccination. In addition, the cytokine patterns of specific T cells during acute H1N1 infection and after vaccination differ, and the predominantly dual-positive cytokine profile of vaccine-induced T cells suggests sufficient functionality to confer successful virus control.<br /> (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Adult
Antibodies, Viral blood
Cross Reactions immunology
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Humans
Immunity, Cellular immunology
Influenza, Human epidemiology
Influenza, Human prevention & control
Influenza, Human virology
Interferon-gamma blood
Interleukin-2 blood
Middle Aged
Neutralization Tests
Statistics, Nonparametric
CD4-Positive T-Lymphocytes immunology
Influenza A Virus, H1N1 Subtype immunology
Influenza Vaccines administration & dosage
Influenza Vaccines immunology
Influenza, Human immunology
Pandemics prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1521-4141
- Volume :
- 42
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 22585549
- Full Text :
- https://doi.org/10.1002/eji.201242393