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Direct evidence that HSV DNA damaged by ultraviolet (UV) irradiation can be repaired in a cell type-dependent manner.
- Source :
-
Journal of neurovirology [J Neurovirol] 2012 Jun; Vol. 18 (3), pp. 231-43. Date of Electronic Publication: 2012 May 12. - Publication Year :
- 2012
-
Abstract
- Infection of permissive cells, in tissue culture, with herpes simplex virus (HSV) has been reported to induce host DNA damage repair responses that are necessary for efficient viral replication. However, direct repair of the damaged viral DNA has not, to our knowledge, been shown. In this report, we detect and determine the amount of damaged HSV-1 DNA, following introduction of experimentally damaged HSV genomes into tissue cultures of permissive Vero, NGF differentiated PC12 cells and primary rat neurons, using a method of detection introduced here. The results show that HSV-1 strain 17 DNA containing UV-induced DNA damage is efficiently repaired, in Vero, but not NGF differentiated PC12 cells. The primary rat neuronal cultures were capable of repairing the damaged viral DNA, but with much less efficiency than did the permissive Vero cells. Moreover, by conducting the experiments with either an inhibitor of the HSV polymerase (phosphonoacetic acid [PAA]) or with a replication defective DNA polymerase mutant virus, HP66, the results suggest that repair can occur in the absence of a functional viral polymerase, although polymerase function seems to enhance the efficiency of the repair, in a replication independent manner. The possible significance of varying cell type mediated repair of viral DNA to viral pathogenesis is discussed.
- Subjects :
- Animals
Chlorocebus aethiops
DNA, Viral genetics
DNA, Viral radiation effects
DNA-Directed DNA Polymerase genetics
DNA-Directed DNA Polymerase metabolism
Enzyme Inhibitors pharmacology
Herpes Simplex genetics
Herpes Simplex virology
Host Specificity
Host-Pathogen Interactions
Neurons pathology
Neurons virology
Nucleic Acid Synthesis Inhibitors
PC12 Cells
Phosphonoacetic Acid pharmacology
Primary Cell Culture
Rats
Simplexvirus metabolism
Ultraviolet Rays
Vero Cells
Virus Replication
DNA Damage
DNA Repair
DNA, Viral metabolism
Herpes Simplex metabolism
Neurons metabolism
Simplexvirus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1538-2443
- Volume :
- 18
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of neurovirology
- Publication Type :
- Academic Journal
- Accession number :
- 22581427
- Full Text :
- https://doi.org/10.1007/s13365-012-0105-2