Resonance Energy Transfer between protein and rhamnolipid capped ZnS quantum dots: application in in-gel staining of proteins.

Authors :: Janakiraman N
Mohan A
Kannan A
Pennathur G
Source :: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2012 Sep; Vol. 95, pp. 478-82. Date of Electronic Publication: 2012 Apr 23.
Publication Year :: 2012
Abstract: The interaction of proteins with quantum dots is an interesting field of research. These interactions occur at the nanoscale. We have probed the interaction of Bovine Serum Albumin (BSA) and Candida rugosa lipase (CRL) with rhamnolipid capped ZnS (RhlZnSQDs) using absorption and fluorescence spectroscopy. Optical studies on mixtures of RhlZnSQDs and proteins resulted in Förster's Resonance Energy Transfer (FRET) from proteins to QDs. This phenomenon has been exploited to detect proteins in agarose gel electrophoresis. The activity of the CRL was unaffected on the addition of QDs as revealed by zymography.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)

Subjects :: Animals
Candida enzymology
Cattle
Spectrometry, Fluorescence
Staining and Labeling
Ultraviolet Rays
Electrophoresis, Agar Gel methods
Energy Transfer
Glycolipids metabolism
Lipase metabolism
Quantum Dots
Serum Albumin, Bovine metabolism
Sulfides metabolism
Zinc Compounds metabolism

Language :: English
ISSN :: 1873-3557
Volume :: 95
Database :: MEDLINE
Journal :: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Publication Type :: Academic Journal
Accession number :: 22580141
Full Text :: https://doi.org/10.1016/j.saa.2012.04.025

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