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Mutation in Torenia fournieri Lind. UFO homolog confers loss of TfLFY interaction and results in a petal to sepal transformation.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2012 Sep; Vol. 71 (6), pp. 1002-14. Date of Electronic Publication: 2012 Jul 09. - Publication Year :
- 2012
-
Abstract
- We identified a Torenia fournieri Lind. mutant (no. 252) that exhibited a sepaloid phenotype in which the second whorls were changed to sepal-like organs. This mutant had no stamens, and the floral organs consisted of sepals and carpels. Although the expression of a torenia class B MADS-box gene, GLOBOSA (TfGLO), was abolished in the 252 mutant, no mutation of TfGLO was found. Among torenia homologs such as APETALA1 (AP1), LEAFY (LFY), and UNUSUAL FLORAL ORGANS (UFO), which regulate expression of class B genes in Arabidopsis, only accumulation of the TfUFO transcript was diminished in the 252 mutant. Furthermore, a missense mutation was found in the coding region of the mutant TfUFO. Intact TfUFO complemented the mutant phenotype whereas mutated TfUFO did not; in addition, the transgenic phenotype of TfUFO-knockdown torenias coincided with the mutant phenotype. Yeast two-hybrid analysis revealed that the mutated TfUFO lost its ability to interact with TfLFY protein. In situ hybridization analysis indicated that the transcripts of TfUFO and TfLFY were partially accumulated in the same region. These results clearly demonstrate that the defect in TfUFO caused the sepaloid phenotype in the 252 mutant due to the loss of interaction with TfLFY.<br /> (© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.)
- Subjects :
- Amino Acid Sequence
Flowers genetics
Flowers growth & development
Flowers radiation effects
Gene Expression Regulation, Plant
Gene Knockdown Techniques
Genetic Complementation Test
In Situ Hybridization
MADS Domain Proteins metabolism
Molecular Sequence Data
Mutation, Missense
Phenotype
Phylogeny
Plant Proteins genetics
Plant Proteins metabolism
Plants, Genetically Modified
Protein Interaction Mapping
Sequence Alignment
Tracheophyta anatomy & histology
Tracheophyta growth & development
Tracheophyta radiation effects
Two-Hybrid System Techniques
Flowers anatomy & histology
MADS Domain Proteins genetics
Tracheophyta genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1365-313X
- Volume :
- 71
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 22577962
- Full Text :
- https://doi.org/10.1111/j.1365-313X.2012.05047.x