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Transplanted mesoangioblasts require macrophage IL-10 for survival in a mouse model of muscle injury.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2012 Jun 15; Vol. 188 (12), pp. 6267-77. Date of Electronic Publication: 2012 May 09. - Publication Year :
- 2012
-
Abstract
- The aim of this study was to verify whether macrophages influence the fate of transplanted mesoangioblasts--vessel-associated myogenic precursors--in a model of sterile toxin-induced skeletal muscle injury. We have observed that in the absence of macrophages, transplanted mesoangioblasts do not yield novel fibers. Macrophages retrieved from skeletal muscles at various times after injury display features that resemble those of immunoregulatory macrophages. Indeed, they secrete IL-10 and express CD206 and CD163 membrane receptors and high amounts of arginase I. We have reconstituted the muscle-associated macrophage population by injecting polarized macrophages before mesoangioblast injection: alternatively activated, immunoregulatory macrophages only support mesoangioblast survival and function. This action depends on the secretion of IL-10 in the tissue. Our results reveal an unanticipated role for tissue macrophages in mesoangioblast function. Consequently, the treatment of muscle disorders with mesoangioblasts should take into consideration coexisting inflammatory pathways, whose activation may prove crucial for its success.
- Subjects :
- Animals
Blotting, Western
Cell Differentiation
Cell Separation
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Fluorescent Antibody Technique
Interleukin-10 immunology
Macrophages immunology
Mice
Mice, Inbred C57BL
Muscle Fibers, Skeletal metabolism
Pericytes metabolism
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Stem Cell Transplantation
Stem Cells metabolism
Interleukin-10 metabolism
Macrophages metabolism
Muscle Fibers, Skeletal cytology
Muscle, Skeletal injuries
Pericytes cytology
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 188
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 22573810
- Full Text :
- https://doi.org/10.4049/jimmunol.1102680