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Studies on carbohydrate xenoantigens.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 885, pp. 47-56. - Publication Year :
- 2012
-
Abstract
- Naturally occurring and elicited anti-carbohydrate antibodies play a major role in immune responses to xenografts. The original obstacles associated with the Gal antigen have been largely resolved by the generation of knockout pigs. In contrast, much less is known about the nature and role of non-Gal carbohydrate antigens and the antibodies recognizing these. These antibodies can be identified and characterized by enzyme-linked immunosorbent assay. Furthermore, the biological significance of the non-Gal antigen(s) can be determined by expression of the relevant glycosyltransferase(s) by transfection and analyzed by antibody and/or lectin binding.
- Subjects :
- Animals
Antigens, Heterophile chemistry
Carbohydrates chemistry
Cell Line
Flow Cytometry
Galactosyltransferases chemistry
Galactosyltransferases genetics
Galactosyltransferases metabolism
Gene Expression
Humans
Immunohistochemistry
Lectins metabolism
Protein Binding
Staining and Labeling
Swine
Transfection
Transplantation, Heterologous immunology
Antigens, Heterophile immunology
Carbohydrates immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 885
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 22565989
- Full Text :
- https://doi.org/10.1007/978-1-61779-845-0_4