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Effects of nitrogen dioxide and anoxia on global gene and protein expression in long-term continuous cultures of Nitrosomonas eutropha C91.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2012 Jul; Vol. 78 (14), pp. 4788-94. Date of Electronic Publication: 2012 May 04. - Publication Year :
- 2012
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Abstract
- Nitrosomonas eutropha is an ammonia-oxidizing betaproteobacterium found in environments with high ammonium levels, such as wastewater treatment plants. The effects of NO(2) on gene and protein expression under oxic and anoxic conditions were determined by maintaining N. eutropha strain C91 in a chemostat fed with ammonium under oxic, oxic-plus-NO(2), and anoxic-plus-NO(2) culture conditions. Cells remained viable but ceased growing under anoxia; hence, the chemostat was switched from continuous to batch cultivation to retain biomass. After several weeks under each condition, biomass was harvested for total mRNA and protein isolation. Exposure of N. eutropha C91 to NO(2) under either oxic or anoxic conditions led to a decrease in proteins involved in N and C assimilation and storage and an increase in proteins involved in energy conservation, including ammonia monooxygenase (AmoCAB). Exposure to anoxia plus NO(2) resulted in increased representation of proteins and transcripts reflective of an energy-deprived state. Several proteins implicated in N-oxide metabolism were expressed and remained unchanged throughout the experiment, except for NorCB nitric oxide reductase, which was not detected in the proteome. Rather, NorY nitric oxide reductase was expressed under oxic-plus-NO(2) and anoxic-plus-NO(2) conditions. The results indicate that exposure to NO(2) results in an energy-deprived state of N. eutropha C91 and that anaerobic growth could not be supported with NO(2) as an oxidant.
- Subjects :
- Aerobiosis
Anaerobiosis
Bacterial Proteins genetics
Bacterial Proteins metabolism
Culture Media chemistry
Microbial Viability
Nitrosomonas classification
Nitrosomonas drug effects
Proteomics
Quaternary Ammonium Compounds metabolism
Time Factors
Gene Expression Profiling
Gene Expression Regulation, Bacterial drug effects
Nitrogen Dioxide pharmacology
Nitrosomonas growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5336
- Volume :
- 78
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 22562996
- Full Text :
- https://doi.org/10.1128/AEM.00668-12