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The order Bacillales hosts functional homologs of the worrisome cfr antibiotic resistance gene.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2012 Jul; Vol. 56 (7), pp. 3563-7. Date of Electronic Publication: 2012 Apr 30. - Publication Year :
- 2012
-
Abstract
- The cfr gene encodes the Cfr methyltransferase that methylates a single adenine in the peptidyl transferase region of bacterial ribosomes. The methylation provides resistance to several classes of antibiotics that include drugs of clinical and veterinary importance. This paper describes a first step toward elucidating natural residences of the worrisome cfr gene and functionally similar genes. Three cfr-like genes from the order Bacillales were identified from BLAST searches and cloned into plasmids under the control of an inducible promoter. Expression of the genes was induced in Escherichia coli, and MICs for selected antibiotics indicate that the cfr-like genes confer resistance to PhLOPSa (phenicol, lincosamide, oxazolidinone, pleuromutilin, and streptogramin A) antibiotics in the same way as the cfr gene. In addition, modification at A2503 on 23S rRNA was confirmed by primer extension. Finally, expression of the Cfr-like proteins was verified by SDS gel electrophoresis of whole-cell extracts. The work shows that cfr-like genes exist in the environment and that Bacillales are natural residences of cfr-like genes.
- Subjects :
- Bacillales metabolism
Bacterial Proteins genetics
Bacterial Proteins metabolism
Diterpenes pharmacology
Drug Resistance, Microbial
Electrophoresis, Polyacrylamide Gel
Escherichia coli drug effects
Lincosamides pharmacology
Microbial Sensitivity Tests
Oxazolidinones pharmacology
Polycyclic Compounds
Streptogramin A pharmacology
Pleuromutilins
Anti-Bacterial Agents pharmacology
Bacillales drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1098-6596
- Volume :
- 56
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 22547628
- Full Text :
- https://doi.org/10.1128/AAC.00673-12