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Accumulation of activated invariant natural killer T cells in the tumor microenvironment after α-galactosylceramide-pulsed antigen presenting cells.
- Source :
-
Journal of clinical immunology [J Clin Immunol] 2012 Oct; Vol. 32 (5), pp. 1071-81. Date of Electronic Publication: 2012 Apr 26. - Publication Year :
- 2012
-
Abstract
- Purpose: The intravenous administration of α-Galactosylceramide (α-GalCer)-pulsed antigen presenting cells (APCs) is well tolerated and the increased IFN-γ producing cells in the peripheral blood after the treatment appeared to be associated with prolonged survival. An exploratory study protocol was designed with the preoperative administration of α-GalCer-pulsed APCs to clarify the mechanisms of these findings, while especially focusing on the precise tumor site.<br />Methods: Patients with operable advanced lung cancer received an intravenous injection of α-GalCer-pulsed APCs before surgery. The resected lung and tumor infiltrating lymphocytes (TILs) as well as peripheral blood mononuclear cells were collected and the invariant NKT (iNKT) cell-specific immune responses were analyzed.<br />Results: Four patients completed the study protocol. We observed a significant increase in iNKT cell numbers in the TILs and augmented IFN-γ production by the α-GalCer-stimulated TILs.<br />Conclusion: The administration of α-GalCer-pulsed APCs successfully induced the dramatic infiltration and activation of iNKT cells in the tumor microenvironment.
- Subjects :
- Adenocarcinoma immunology
Adenocarcinoma therapy
Aged
Carcinoma, Non-Small-Cell Lung immunology
Carcinoma, Squamous Cell immunology
Carcinoma, Squamous Cell therapy
Humans
Lung Neoplasms immunology
Lymph Nodes immunology
Male
Receptors, Antigen, T-Cell genetics
Receptors, Antigen, T-Cell immunology
Tumor Microenvironment immunology
Antigen-Presenting Cells immunology
Carcinoma, Non-Small-Cell Lung therapy
Galactosylceramides
Immunotherapy
Lung Neoplasms therapy
Natural Killer T-Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-2592
- Volume :
- 32
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of clinical immunology
- Publication Type :
- Academic Journal
- Accession number :
- 22534863
- Full Text :
- https://doi.org/10.1007/s10875-012-9697-9