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Microchip-based immunoassays with application of silicon dioxide nanoparticle film.
- Source :
-
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2012 Jun; Vol. 403 (8), pp. 2449-57. Date of Electronic Publication: 2012 Apr 24. - Publication Year :
- 2012
-
Abstract
- Highly sensitive detection of proteins offers the possibility of early and rapid diagnosis of various diseases. Microchip-based immunoassay integrates the benefits from both immunoassays (high specificity of target sample) and microfluidics (fast analysis and low sample consumption). However, direct capture of proteins on bare microchannel surface suffers from low sensitivity due to the low capacity of microsystem. In this study, we demonstrated a microchip-based heterogeneous immunoassay using functionalized SiO(2) nanoparticles which were covalently assembled on the surface of microchannels via a liquid-phase deposition technique. The formation of covalent bonds between SiO(2) nanoparticles and polydimethylsiloxane substrate offered sufficient stability of the microfluidic surface, and furthermore, substantially enhanced the protein capturing capability, mainly due to the increased surface-area-to-volume ratio. IgG antigen and FITC-labeled anti-IgG antibody conjugates were adopted to compare protein-enrichment effect, and the fluorescence signals were increased by ~75-fold after introduction of functionalized SiO(2) nanoparticles film. Finally, a proof-of-concept experiment was performed by highly efficient capture and detection of inactivated H1N1 influenza virus using a microfluidic chip comprising highly ordered SiO(2) nanoparticles coated micropillars array. The detection limit of H1N1 virus antigen was 0.5 ng mL(-1), with a linear range from 20 to 1,000 ng mL(-1) and mean coefficient of variance of 4.71%.
- Subjects :
- Animals
Antibodies, Anti-Idiotypic analysis
Antibodies, Anti-Idiotypic immunology
Equipment Design
Fluorescein-5-isothiocyanate analysis
Goats
Immunoglobulin G immunology
Influenza A Virus, H1N1 Subtype immunology
Nanoparticles ultrastructure
Orthomyxoviridae Infections immunology
Rabbits
Immunoassay instrumentation
Influenza A Virus, H1N1 Subtype isolation & purification
Microfluidic Analytical Techniques instrumentation
Nanoparticles chemistry
Orthomyxoviridae Infections diagnosis
Silicon Dioxide chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1618-2650
- Volume :
- 403
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 22526636
- Full Text :
- https://doi.org/10.1007/s00216-012-5952-4