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Glioma immunotherapy with combined autologous tumor cell and endothelial cell vaccine in vivo.
- Source :
-
Neurologia medico-chirurgica [Neurol Med Chir (Tokyo)] 2012; Vol. 52 (4), pp. 194-201. - Publication Year :
- 2012
-
Abstract
- Combined vaccines containing GL261 murine glioma cells and F-2 murine endothelial cells fixed with glutaraldehyde-phosphate buffered saline were injected into the intradermal tissue of the tail base of C57BL/6 mice. After the vaccination, GL261 cells were injected subcutaneously into the left flank of the mice. Vaccination with fixed F-2 cells induced the development of relatively high amounts of interferon-gamma-releasing cells after in vitro re-stimulation with vascular endothelial growth factor-receptor 2 peptide. Tumor growth was inhibited after preventive use of the combined vaccine, prepared from GL261 and F-2 cells. Tumor specimens obtained from the combined vaccine group in a therapeutic experiment showed significantly decreased vessel count. Glioma immunotherapy with a combined vaccine prepared from tumor cells and endothelial cells might represent a new clinical strategy, as such combinations may theoretically affect both high-grade glioma cells and their environment.
- Subjects :
- Animals
Brain Neoplasms blood supply
Brain Neoplasms immunology
Cancer Vaccines immunology
Cell Line, Tumor
Glioma blood supply
Glioma immunology
Immunotherapy methods
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Neoplasm Transplantation methods
Neoplasms, Experimental immunology
Neoplasms, Experimental physiopathology
Primary Cell Culture
Vaccines, Combined immunology
Brain Neoplasms therapy
Cancer Vaccines pharmacology
Cell Transplantation methods
Glioma therapy
Neoplasms, Experimental therapy
Vaccines, Combined pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1349-8029
- Volume :
- 52
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Neurologia medico-chirurgica
- Publication Type :
- Academic Journal
- Accession number :
- 22522329
- Full Text :
- https://doi.org/10.2176/nmc.52.194