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Heterogeneity across the dorso-ventral axis in zebrafish EVL is regulated by a novel module consisting of sox, snail1a and max genes.
- Source :
-
Mechanisms of development [Mech Dev] 2012 Mar-Jun; Vol. 129 (1-4), pp. 13-23. Date of Electronic Publication: 2012 Apr 12. - Publication Year :
- 2012
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Abstract
- In vertebrates, the dorso-ventral (DV) axis is defined by the combinatorial action of localised Wnt, FGF and Nodal signalling along with the antagonizing activities of Chordin and BMP pathways. Our knowledge of the factors that may act in concert with these core pathways to regulate early embryonic patterning is far from complete. Furthermore, while all three germ layers respond to these patterning cues, it is not clear whether in zebrafish the outermost protective epithelium, the enveloping layer (EVL), is also patterned along the DV axis. Here, we have identified a transgenic line driving GFP under a crestin promoter, which specifically labels the dorsal domain of the EVL suggesting heterogeneity in the EVL across the DV axis. Our attempts to understand how the expression from this promoter fragment is regulated specifically in the dorsal domain, have unravelled potential novel players involved in early EVL and embryonic patterning. We show that along with Nodal signalling components, four proteins Sox11b, Sox19b, Snail1a and Max are involved in regulating the size of this EVL domain. However, Chordin-BMP signalling might be dispensable for the dorso-ventral patterning of the EVL. For the first time, this transgenic line unravels the heterogeneity in the EVL and will serve as an important tool in understanding the molecular basis of the DV patterning of the EVL.<br /> (Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Animals, Genetically Modified
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors metabolism
Body Patterning
Bone Morphogenetic Protein 2 physiology
Embryo, Nonmammalian cytology
Embryo, Nonmammalian metabolism
Gene Expression
Gene Expression Regulation, Developmental
Glycoproteins metabolism
Intercellular Signaling Peptides and Proteins metabolism
Promoter Regions, Genetic
SOX Transcription Factors metabolism
Signal Transduction
Snail Family Transcription Factors
Transcription Factors metabolism
Zebrafish genetics
Zebrafish metabolism
Zebrafish Proteins metabolism
Zebrafish Proteins physiology
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors genetics
SOX Transcription Factors genetics
Transcription Factors genetics
Zebrafish embryology
Zebrafish Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1872-6356
- Volume :
- 129
- Issue :
- 1-4
- Database :
- MEDLINE
- Journal :
- Mechanisms of development
- Publication Type :
- Academic Journal
- Accession number :
- 22522081
- Full Text :
- https://doi.org/10.1016/j.mod.2012.03.003