Development of reagents to study the turkey's immune response: cloning and characterisation of two turkey cytokines, interleukin (IL)-10 and IL-13.

Authors :: Powell F
Rothwell L
Clarkson M
Kaiser P
Source :: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2012 Jun 15; Vol. 147 (1-2), pp. 97-103. Date of Electronic Publication: 2012 Mar 30.
Publication Year :: 2012
Abstract: The cDNAs of two turkey cytokines, interleukin (IL)-10 and IL-13, were cloned using oligonucleotide primers designed from their chicken orthologues. The coding regions of the chicken and turkey genes are highly conserved, with IL-10 and IL-13 exhibiting 94.1% and 90% nucleotide and 92% and 79.9% amino acid identity respectively. Both showed consistent mRNA expression in turkey lymphoid and gut tissues. Expression in non-lymphoid tissues was more variable but generally highest in the skin and trachea. Recombinant turkey IL-10 was expressed and bioactivity demonstrated by inhibition of IFN-γ synthesis from activated splenocytes. Chicken and turkey IL-10 cross-reacted in functional assays.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)

Subjects :: Amino Acid Sequence
Animals
Avian Proteins chemistry
Chickens
Cloning, Molecular
Cross Reactions
Indicators and Reagents
Interleukin-10 chemistry
Interleukin-13 chemistry
Molecular Sequence Data
Recombinant Proteins biosynthesis
Avian Proteins genetics
Interleukin-10 genetics
Interleukin-13 genetics
Turkeys immunology

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