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Proteinase-activated receptor-4 plays a major role in the recruitment of neutrophils induced by trypsin or carrageenan during pleurisy in mice.
- Source :
-
Pharmacology [Pharmacology] 2012; Vol. 89 (5-6), pp. 275-82. Date of Electronic Publication: 2012 Apr 17. - Publication Year :
- 2012
-
Abstract
- Background/aims: The activation of proteinase-activated receptors (PARs) has been implicated in the development of important hallmarks of inflammation, including in vivo leukocyte recruitment. Here, we examined the effects of aprotinin, a potent inhibitor of trypsin proteinase and the kallikrein-kinin system, and the PAR-4 antagonist YPGKF-NH(2) (tcY-NH(2)) on neutrophil recruitment in response to carrageenan and trypsin in the pleural cavity of mice.<br />Methods: BALB/c mice were intrapleurally injected with trypsin or PAR-4-activating peptide AY-NH(2), pretreated with aprotinin or tcY-NH(2) (1 μg/cavity) prior to an intrapleural injection of trypsin or carrageenan, or pretreated with leukotriene B(4) antagonist U-75302 (3 μg/cavity) prior to a trypsin injection. The number of infiltrating neutrophils was evaluated after 4 h.<br />Results: PAR-4-activating peptide AY-NH(2) and trypsin-induced neutrophil recruitment was inhibited by aprotinin, tcY-NH(2) or U-75302. Aprotinin and tcY-NH(2) also inhibited neutrophil recruitment induced by carrageenan.<br />Conclusion: These data suggest a key role for PAR-4 in mediating neutrophil recruitment in a mouse model of pleurisy induced by the activity of trypsin or trypsin-like enzymes.<br /> (Copyright © 2012 S. Karger AG, Basel.)
- Subjects :
- Animals
Aprotinin pharmacology
Carrageenan
Cell Movement drug effects
Disease Models, Animal
Female
Mice
Mice, Inbred BALB C
Neutrophils drug effects
Oligopeptides pharmacology
Pleurisy chemically induced
Receptors, Proteinase-Activated antagonists & inhibitors
Trypsin
Trypsin Inhibitors pharmacology
Neutrophils immunology
Pleurisy immunology
Receptors, Proteinase-Activated immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0313
- Volume :
- 89
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 22517275
- Full Text :
- https://doi.org/10.1159/000337378