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Glucose transport by acinar cells in rat parotid glands.
- Source :
-
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2012; Vol. 29 (3-4), pp. 325-30. Date of Electronic Publication: 2012 Apr 03. - Publication Year :
- 2012
-
Abstract
- Background/aims: Salivary glucose is often considered as being from glandular origin. Little information is available, however, on the possible role of glucose transporters in the secretion of the hexose by salivary glands. The major aim of the present study was to investigate the expression and localization of several distinct glucose transporters in acinar cells of rat parotid glands.<br />Methods: Quantitative real-time PCR analysis, immunohistochemistry and western blotting techniques were used to assess the presence of SGLT1, GLUT1, GLUT2 and GLUT4 in acinar cells of rat parotid glands.<br />Results: Quantitative real-time PCR documented the expression of SGLT1 and GLUT1 in parotid tissues, with a much lower level of GLUT4 mRNA and no expression of GLUT2 mRNA. Western blot analysis revealed the presence of SGLT1, GLUT1 and GLUT4 proteins, but not GLUT2 proteins in the parotid extract. Immunohistochemistry confirmed these findings. SGLT1 was specifically located at the baso-lateral membrane, co-localizing with Na(+)/K(+) ATPase. GLUT1 was found both at the baso-lateral and apical level. GLUT4 appeared to be also located at the baso-lateral level. However, too little GLUT4 was present to allow co-localization labeling.<br />Conclusion: Based on these findings, a model is proposed for the transport of glucose into the acinar cells and thereafter into the acinar lumen.<br /> (Copyright © 2012 S. Karger AG, Basel.)
- Subjects :
- Acinar Cells cytology
Animals
Biological Transport
Cell Membrane metabolism
Female
Gene Expression Regulation
Glucose Transport Proteins, Facilitative genetics
Glucose Transporter Type 1 genetics
Glucose Transporter Type 1 metabolism
Glucose Transporter Type 2 genetics
Glucose Transporter Type 2 metabolism
Immunohistochemistry
Kidney cytology
Kidney metabolism
Pancreas cytology
Pancreas metabolism
Parotid Gland cytology
RNA, Messenger genetics
RNA, Messenger metabolism
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Sodium-Glucose Transporter 1 genetics
Sodium-Potassium-Exchanging ATPase metabolism
Acinar Cells metabolism
Glucose metabolism
Glucose Transport Proteins, Facilitative metabolism
Parotid Gland metabolism
Sodium-Glucose Transporter 1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1421-9778
- Volume :
- 29
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 22508040
- Full Text :
- https://doi.org/10.1159/000338487