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In vivo participation of nitric oxide in hyperproliferative epidermal phenomena in mice.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2012 Jul 15; Vol. 687 (1-3), pp. 1-8. Date of Electronic Publication: 2012 Apr 03. - Publication Year :
- 2012
-
Abstract
- A significant involvement of nitric oxide (NO) in the process of keratinocyte proliferation is reported with many divergences. To determine the involvement of NO in the hyperproliferative process of epidermis in vivo, non-selective inhibitor (N(G)-nitro-L-arginine-methyl ester.HCl: L-NAME) and selective inhibitors for inducible NO synthase (iNOS) and neuronal NO synthase (nNOS) (Aminoguanidine: AG and 7-Nitroindazole: 7-NI, respectively) and a NO-donor (Sodium nitroprusside: SNP) were topically applied twice a day in mice ear treated with multiple applications of croton oil. L-NAME and 7-NI treatments decreased and SNP increased ear edema formation. However, ear weight was reduced in groups that received L-NAME and 7-NI, while the AG and SNP groups presented an increment. The histological evaluation of epidermis thickness showed that all NOS inhibitors were able to prevent the increase in epidermis width caused by croton oil, while SNP contributed to enlargement. The same results were observed in the PCNA staining, where treatments with NOS inhibitors caused a reduction in the number of cells in the epidermis, while SNP caused an enhancement. 7-NI treatment reduced polymorphonuclear and mononuclear leukocytes migration when compared to the control group. The AG application increased the migration of polymorphonuclear and mononuclear cells, while the SNP enhanced only the polymorphonuclear cells. Therefore, in the skin NO produced by nNOS is involved in the control of keratinocyte hyperproliferation, with the contribution of iNOS. In the animal model of cutaneous chronic inflammation by croton oil, NO is involved in the exudation and leukocyte migration, with participation of all three enzymes.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Acetylglucosaminidase metabolism
Animals
Anti-Inflammatory Agents pharmacology
Cell Proliferation drug effects
Croton Oil
Dermatitis, Contact etiology
Dermatitis, Contact pathology
Dexamethasone pharmacology
Disease Models, Animal
Enzyme Inhibitors pharmacology
Epidermis drug effects
Epidermis metabolism
Guanidines pharmacology
Indazoles pharmacology
Keratinocytes drug effects
Male
Mice
NG-Nitroarginine Methyl Ester pharmacology
Nitric Oxide Donors pharmacology
Nitric Oxide Synthase Type I antagonists & inhibitors
Nitric Oxide Synthase Type II antagonists & inhibitors
Nitroprusside pharmacology
Peroxidase metabolism
Dermatitis, Contact metabolism
Epidermis pathology
Keratinocytes pathology
Nitric Oxide metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0712
- Volume :
- 687
- Issue :
- 1-3
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 22498002
- Full Text :
- https://doi.org/10.1016/j.ejphar.2012.03.041