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A novel anticancer and antifungus phenazine derivative from a marine actinomycete BM-17.

Authors :
Gao X
Lu Y
Xing Y
Ma Y
Lu J
Bao W
Wang Y
Xi T
Source :
Microbiological research [Microbiol Res] 2012 Dec 20; Vol. 167 (10), pp. 616-22. Date of Electronic Publication: 2012 Apr 10.
Publication Year :
2012

Abstract

A marine actinomycete, designated strain BM-17, was isolated from a sediment sample collected in the Arctic Ocean. The strain was identified as Nocardia dassonvillei based on morphological, cultural, physiological, biochemical characteristics, along with the cell wall analysis and 16S rDNA gene sequence analysis. A new secondary metabolite (1), N-(2-hydroxyphenyl)-2-phenazinamine (NHP), and six known antibiotics (2-7) have been isolated from the saline culture broth of the stain by sequentially purification over macroporous resin D101, silica gel, Sephadex LH-20 column chromatography and preparative HPLC after the stain was incubated in soy bean media at 28°C for 7 days. The chemical structures of the compounds were elucidated on the basis of spectroscopic analysis, including two-dimensional (2D) NMR and HR-ESI-MS data. The new compound showed significant antifungal activity against Candida albicans, with a MIC of 64 μg/ml and high cancer cell cytotoxicity against HepG2, A549, HCT-116 and COC1 cells.<br /> (Copyright © 2012 Elsevier GmbH. All rights reserved.)

Details

Language :
English
ISSN :
1618-0623
Volume :
167
Issue :
10
Database :
MEDLINE
Journal :
Microbiological research
Publication Type :
Academic Journal
Accession number :
22494896
Full Text :
https://doi.org/10.1016/j.micres.2012.02.008