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Myosin light-chain kinase is necessary for membrane homeostasis in cochlear inner hair cells.
- Source :
-
PloS one [PLoS One] 2012; Vol. 7 (4), pp. e34894. Date of Electronic Publication: 2012 Apr 02. - Publication Year :
- 2012
-
Abstract
- The structural homeostasis of the cochlear hair cell membrane is critical for all aspects of sensory transduction, but the regulation of its maintenance is not well understood. In this report, we analyzed the cochlear hair cells of mice with specific deletion of myosin light chain kinase (MLCK) in inner hair cells. MLCK-deficient mice showed impaired hearing, with a 5- to 14-dB rise in the auditory brainstem response (ABR) thresholds to clicks and tones of different frequencies and a significant decrease in the amplitude of the ABR waves. The mutant inner hair cells produced several ball-like structures around the hair bundles in vivo, indicating impaired membrane stability. Inner hair cells isolated from the knockout mice consistently displayed less resistance to hypoosmotic solution and less membrane F-actin. Myosin light-chain phosphorylation was also reduced in the mutated inner hair cells. Our results suggest that MLCK is necessary for maintaining the membrane stability of inner hair cells.
- Subjects :
- Actin Cytoskeleton metabolism
Actins metabolism
Animals
Cell Membrane metabolism
Epithelium enzymology
Epithelium metabolism
Evoked Potentials, Auditory, Brain Stem
Gene Expression
Hair Cells, Auditory, Inner metabolism
Hair Cells, Auditory, Inner ultrastructure
Mice
Mice, 129 Strain
Mice, Inbred C57BL
Mice, Knockout
Myosin Light Chains metabolism
Myosin VIIa
Myosin-Light-Chain Kinase deficiency
Myosin-Light-Chain Kinase genetics
Myosins metabolism
Organ of Corti cytology
Osmotic Pressure
Phosphorylation
Protein Processing, Post-Translational
Sequence Deletion
Sodium-Potassium-Exchanging ATPase genetics
Sodium-Potassium-Exchanging ATPase metabolism
Cell Membrane enzymology
Hair Cells, Auditory, Inner enzymology
Homeostasis
Myosin-Light-Chain Kinase physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 7
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 22485190
- Full Text :
- https://doi.org/10.1371/journal.pone.0034894