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Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1.
- Source :
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Genetics and molecular biology [Genet Mol Biol] 2012 Jan; Vol. 35 (1), pp. 164-71. Date of Electronic Publication: 2012 Feb 16. - Publication Year :
- 2012
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Abstract
- Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.
Details
- Language :
- English
- ISSN :
- 1678-4685
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genetics and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 22481891
- Full Text :
- https://doi.org/10.1590/s1415-47572012005000021