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A new experimental polytrauma model in rats: molecular characterization of the early inflammatory response.
- Source :
-
Mediators of inflammation [Mediators Inflamm] 2012; Vol. 2012, pp. 890816. Date of Electronic Publication: 2012 Feb 29. - Publication Year :
- 2012
-
Abstract
- Background: The molecular mechanisms of the immune response after polytrauma are highly complex and far from fully understood. In this paper, we characterize a new standardized polytrauma model in rats based on the early molecular inflammatory and apoptotic response.<br />Methods: Male Wistar rats (250 g, 6-10/group) were anesthetized and exposed to chest trauma (ChT), closed head injury (CHI), or Tib/Fib fracture including a soft tissue trauma (Fx + STT) or to the following combination of injuries: (1) ChT; (2) ChT + Fx + STT; (3) ChT + CHI; (4) CHI; (5) polytrauma (PT = ChT + CHI + Fx + STT). Sham-operated rats served as negative controls. The inflammatory response was quantified at 2 hours and 4 hours after trauma by analysis of "key" inflammatory mediators, including selected cytokines and complement components, in serum and bronchoalveolar (BAL) fluid samples.<br />Results: Polytraumatized (PT) rats showed a significant systemic and intrapulmonary release of cytokines, chemokines, and complement anaphylatoxins, compared to rats with isolated injuries or selected combinations of injuries.<br />Conclusion: This new rat model appears to closely mimic the early immunological response of polytrauma observed in humans and may provide a valid basis for evaluation of the complex pathophysiology and future therapeutic immune modulatory approaches in experimental polytrauma.
- Subjects :
- Animals
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Inflammation metabolism
Interleukin-6 blood
Lung immunology
Lung metabolism
Lung pathology
Male
Multiple Trauma complications
Random Allocation
Rats
Rats, Wistar
Inflammation blood
Inflammation etiology
Multiple Trauma blood
Multiple Trauma immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1861
- Volume :
- 2012
- Database :
- MEDLINE
- Journal :
- Mediators of inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 22481866
- Full Text :
- https://doi.org/10.1155/2012/890816