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Analysis of the targeting properties of Fc chimeric proteins and antibodies in mice in vivo.
- Source :
-
Current protocols in immunology [Curr Protoc Immunol] 2012 Apr; Vol. Chapter 18, pp. Unit 18.18.1-12. - Publication Year :
- 2012
-
Abstract
- This unit includes protocols for the analysis of the targeting properties of Fc chimeric proteins and antibodies in mice in vivo. We present procedures to prepare the reagents to be tested under endotoxin-free conditions, suitable injection sites, methodology for tissue collection, and processing, and labeling procedures to detect the injected material in situ.<br /> (© 2012 by John Wiley & Sons, Inc.)
- Subjects :
- Animals
Antibodies, Monoclonal
Humans
Immunoglobulin Fc Fragments genetics
Immunoglobulin Fc Fragments immunology
Immunoglobulin G immunology
Immunophenotyping
Mice
Rats
Recombinant Fusion Proteins administration & dosage
Recombinant Fusion Proteins analysis
Recombinant Fusion Proteins immunology
Staining and Labeling
Immunoglobulin Fc Fragments administration & dosage
Immunoglobulin Fc Fragments analysis
Immunoglobulin G administration & dosage
Immunoglobulin G analysis
Immunohistochemistry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1934-368X
- Volume :
- Chapter 18
- Database :
- MEDLINE
- Journal :
- Current protocols in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 22470135
- Full Text :
- https://doi.org/10.1002/0471142735.im1818s97