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Terpenoidal constituents of Eucalyptus loxophleba ssp. lissophloia.

Authors :
Sidana J
Singh S
Arora SK
Foley WJ
Singh IP
Source :
Pharmaceutical biology [Pharm Biol] 2012 Jul; Vol. 50 (7), pp. 823-7. Date of Electronic Publication: 2012 Apr 03.
Publication Year :
2012

Abstract

Context: Eucalyptus has been a source of a number of biologically active compounds. The anti-leishmanial activity of terpenoids from Eucalyptus loxophleba (Benth.) ssp. lissophloia (Myrtaceae) has not yet been investigated.<br />Objective: Isolation of the terpenoidal constituents for evaluation of in vitro anti-leishmanial activity against the Leishmania donovani (Dd8 strain) promastigotes.<br />Materials and Methods: The chloroform-methanol (8:2) extract of dried leaves of Eucalyptus loxophleba was used to isolate terpenoidal constituents employing solvent partitioning, column chromatography and preparative high performance liquid chromatography and characterized from spectral data. The anti-leishmanial activity of the isolated compounds was tested in vitro using an Alamar blue assay against a culture of L. donovani (Dd8 strain) promastigotes.<br />Results: Two new naturally occurring triterpenes, named loxanic acid and 3-acetyl loxanic acid together with four known ursane triterpenoids and one bis-monoterpene glycoside, cuniloside B isolated from the leaves showed anti-leishmanial activity (IC(50) 133 to 235 μM) against the promastigotes of the tested strain.<br />Conclusion: The terpenes isolated from the leaves of E. loxophleba showed moderate anti-leishmanial activity.

Details

Language :
English
ISSN :
1744-5116
Volume :
50
Issue :
7
Database :
MEDLINE
Journal :
Pharmaceutical biology
Publication Type :
Academic Journal
Accession number :
22468852
Full Text :
https://doi.org/10.3109/13880209.2011.636058